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*NEW* Viruses and Viroids

This test determines whether a plant sample is contaminated with RNA from any of three (3) separate pathogens. One RNA Collection Kit is included with the price of each test. Each kit collects one sample and you will need one kit for each plant that you want to test. Shipping will be calculated during checkout. All taxes are included.

 

$50.00$99.99

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Use Cases for Virus and Viroid Screening:

Are your plants suffering from “dudding”? That’s a common disease caused by and of several infections resulting in reduced yield and quality of your grow. If your previously healthy plants have suddenly started becoming stunted in growth, then a viral or viroid infection may be the cause.

  • Screening your mother plants
  • Screen new clones before bringing them into your garden
  • Gain peace of mind knowing your prized plants are healthy. 
  • Ensure your remaining plants are healthy after dealing with an infection within your facility. 

How It Works: A single test screens for the presence of Hop Latent Viroid (HLVd), Lettuce Chlorosis Virus (LCV), and Cannabis Cryptic Virus (CCV). These are three viruses commonly found in cannabis and hemp that can impact yield. When testing cannabis we require the use of a special RNA collection kit which we provide, and is included with the cost of testing. Our kits enable you to collect viral RNA from leaves without extracting any cannabinoids; making samples legal to mail back to our lab from anywhere in the world. We validated the diagnostic assay on plants showing symptoms of HLVd infection in a fully operational licensed commercial cultivation facility. This test can specifically and sensitively distinguish HLVd, LCV and CCV from other plant pathogens, even other RNA viruses, that might be causing symptoms of stunted growth. 

We include an internal positive control in every test reaction and negative controls in every batch of tests we run. The positive control provides a known reference so that we can obtain a conclusive result for the specific presence or absence of viral/viroid RNA in a sample. A positive control ensures that negative test results don’t simply arise from poor sample quality or other factors that could prevent successful detection. Negative controls (a “blank” test that doesn’t contain sample material) ensure we don’t generate erroneous false positives due to potential contamination. This extra information is incredibly useful when screening for low levels of contamination.

Detailed instructions along with a sample collection form will be sent to you with the sample collection kits so you can properly document all necessary information. We track samples submitted to our lab using the sample collection form, which should contain the same contact information used when purchasing testing here on our website. Please contact us or include a note with your order if you would like results sent to a different email address than used for payment.

*Important Disclaimer = Sample collection for viruses and viroids requires using a 10% bleach solution, instead of 70% alcohol. MODIFY ALL STEPS IN THE SAMPLE COLLECTION PROTOCOL TO USE 10% BLEACH INSTEAD OF 70% ALCOHOL. This is required to destroy virus particles and prevent spreading infections between your plants. Alcohol will not destroy the virus completely – you MUST use a diluted solution of 1 part bleach to 9 parts water.

Results and Billing

We deliver results electronically within 2-5 business days after we receive your samples. Turnaround time ultimately depends on the number and quality of samples submitted. Charges will appear on your statement as Delta Consulting.

 

Hop Latent Viroid

Hop latent viroid (HLVd) is a single-stranded, circular infectious RNA that completely depends on the metabolism of the host plant. As the name suggests, HLVd was first  identified in the hop plant, but it can also infect cannabis, which is a relative of hops.  In fact, some experts claim the effects of the disease are even more serious in cannabis, causing stunted plants with small, loose buds that have fewer trichomes. Once infected, plants can only be saved via a lengthy tissue culture process that will produce a new viroid-free plant.

How does Hop Latent Viroid affect Cannabis and Hemp Plants?

HLVd won’t kill the plant or even cause obvious symptoms of infection (i.e., curling or yellowing leaves); however plants will show subtle symptoms.

HLVd will greatly reduce the quality and quantity of the flower the infected plant produces.  During the vegetative stage, plants will grow shorter with smaller leaves and tighter node spacing. Flowering plants will have smaller looser buds with much fewer trichomes. One of our clients estimated that plants infected with HLVd had half the cannabinoid content of healthy plants.

 

How does Hop Latent Viroid spread?

HLVd is most commonly spread via infected pruning tools, which is why cultivators should always sterilize their equipment before starting work on a new plant.

HLVd can also spread through cloning when cuttings are taken from an infected mother. And because symptoms of HLVd are not obvious, it can be hard to identify infected mother plants. This is especially true when infection occurs later in the plant’s development, since stunted growth will not be as apparent.

 

How can Hop Latent Viroid be Controlled?

Although HLVd can be eliminated from a cannabis or hemp plant via tissue culture, it is a long and laborious process that should only be reserved for cultivars that are critical to you business.

As with most plant pathogens, prevention is key. Good sanitation practices will go a long way in preventing the spread of HLVd and all other plant pathogens. Use fresh gloves each time you handle a new plant and sterilize tools often.

Cultivators should also screen mother plants with qPCR tests to make sure any cuttings that are taken will be virus free. Cultivators can also screen incoming clones with qPCR assays to make sure they are not introducing infected plants to their grow.

 

Lettuce Chlorosis Virus
Lettuce chlorosis virus (LCV) is a linear, single-stranded positive-sense RNA virus and a member of the Crinivirus family. Cannabis and hemp plants that are infected early will suffer stunted growth, but may not show visual signs until a few weeks into flowering. Those visual signs of LCV infection include yellow, rolling, brittle leaves. While the infection does not kill cannabis and hemp plants, it significantly affects yield and potency. The plant will produce smaller flowers with fewer trichomes, cannabinoids, and terpenes.

How does Lettuce Chlorosis Virus affect Cannabis and Hemp Plants?

Cannabis and hemp plants that are infected early will have stunted growth, but may not show visual signs until a few weeks into flowering. Those visual signs of LCV infection include yellow, rolling, brittle leaves. While the infection does not kill cannabis and hemp plants, it significantly affects yield. Plant will produce smaller flowers with fewer trichomes, cannabinoids, and terpenes.

How does Lettuce Chlorosis Virus spread?

Studies have shown that LCV can be transmitted from plant to plant via pests, specifically the whitefly. There have also been claims that the virus can be transmitted via water.

More commonly, LCV can is spread through cloning when cuttings are taken from an infected mother. And because symptoms of LCV often present in the flowering stage, it can be hard to identify infected mother plants. This is especially true when infection occurs later in the plant’s development, since stunted growth will not be as apparent.

How can Lettuce Chlorosis Virus be Controlled?

Once a cannabis or hemp plant is infected with LCV, it can only be eliminated from the plant via tissue culture. Cultivators should screen mother plants with qPCR tests to make sure any cuttings that are taken will be virus free. If the mother does have LCV, cultivators can use tissue culture to eliminate the virus and produce a new, healthy mother. Cultivators can also screen incoming clones with qPCR assays to make sure they are not introducing infected plants to their grow.

 

Cannabis Cryptic Virus

Cannabis cryptic virus (CCV) is a double-stranded RNA virus and a member of the Partitiviridae family. Researchers detected CCV in leaves showing signs of hemp streak virus; however, the presence of CCV alone did not cause streak symptoms. It’s possible that CCV interacts with other fungal pathogens to produce disease symptoms in cannabis and hemp plants, which is why monitoring the presence of CCV could be valuable. Furthermore, CCV can be vertically transmitted through seeds, which means breeders may want to screen plants before crossing.

*NEW* Viruses and Viroids

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