*NEW* Total Yeast and Mold (TYM)
The results of a Total Yeast and Mold (TYM) test are an indication of yeast and mold contamination on a cannabis sample. One Plant DNA Collection Kit is included with the price of each test. Each kit collects one sample and you will need one kit for each plant that you want to test. Shipping will be calculated during checkout. All taxes are included
Total Yeast and Mold
However, TYM tests are unable to differentiate between pathogenic, beneficial, and benign yeast and molds, making them poor indicators of safety. A low TYM result does not mean a cannabis sample is free of pathogens. A high TYM result doesn’t mean a sample is harmful to consumers. This is especially true in cannabis, which has a diverse microbiome of beneficial microbes that are not harmful to humans. For example, many organic cannabis growers use Trichoderma, a fungus that can help plant nutrition and protect it from disease.
Still, many cannabis regulators require cannabis samples pass TYM testing before they can be sold in dispensaries. The most current version of the Cannabis Inflorescence and Leaf monograph published by the American Herbal Pharmacopoeia, which many states use to determine pass or fail criteria for microbial testing, recommends less than 10,000 CFU/g on cannabis plant material and 1,000 CFU/g on extracts. This test enables us to convert the quantification cycle (Cq) value from the qPCR assay to colony forming units (CFU). In order to maintain the best possible concordance with plating, the TYM assay does not include certain species that fail to culture. Powdery Mildew and Botrytis are two such species and are therefore omitted in the assay design.
Who requires TYM testing?
- New Hampshire
- New Mexico
- North Dakota
- Rhode Island
*Only required for select matrices, view MGC’s Cannabis Microbial Testing Regulations by State page for more information
Why Use qPCR for TYM testing on Cannabis?
Peer-reviewed studies have shown that qPCR testing methods are able to more accurately detect and quantify yeast and mold species present on a cannabis sample than culture-based methods. TYM testing is analogous to a more quantitative version of the internal control built into our Powdery Mildew Screening, because it targets a specific DNA sequence that exists in all yeasts and molds. Because of this, the TYM test can detect all yeast and mold species, regardless of whether they grow in culture. Culture-based tests can only detect the species that grow in a given medium and timeframe. Conversely, qPCR can more effectively exclude off-target microbial species, such as bacteria, which have been shown to grow in TYM culture medium.
Test for Total Yeast and Mold with Confidence
We can test for Total Yeast and Mold in a variety of cannabis matrices. The PathoSEEK® Platform is the only method designed for and validated on cannabis flower, extracts, and a variety of infused products. We proudly partner with Medicinal Genomics to provide several of our validated cannabis testing services.
*For Research Use Only*
We’e developed a simple method to collect microbal DNA samples from your plants. This enables anyone in the world to take advantage of our services because our kits produce a purified DNA sample which is 100% legal to mail. All you need are the same materials (tweezers, alcohol, etc) required for any of our other tests. If you would like to test something other than plant material please contact us before placing your order.
All testing is offered for research purposes only and not valid for regulatory certification.