Hop Latent Viroid (HLVd)
This test determines whether a plant sample is contaminated with RNA from Hop Latent Viroid (HLVd). One RNA Collection Kit is included with the price of each test. Each kit collects one sample and you will need one kit for each plant that you want to test. Shipping will be calculated during checkout. All taxes are included.
Hop Latent Viroid (HLVd)
Purpose and Use Cases
Screen your mother plants.
Screen new clones before adding them to your garden.
Gain peace of mind knowing your prized plants are healthy!
How It Works
Important Disclaimer = Sample collection for HLVd requires using a 10% bleach solution, instead of 70% alcohol. MODIFY ALL STEPS IN THE SAMPLE COLLECTION PROTOCOL TO USE 10% BLEACH INSTEAD OF 70% ALCOHOL. This is required to destroy viroid particles in contaminating plant tissue and prevent spreading infections between your plants. Alcohol will not adequately decontaminate viroid infected tissue – you MUST use a diluted solution of 1 part bleach to 9 parts water.
HLVd Screening works on all plant species. However, particularly when testing cannabis, we require using a special RNA collection kit that we developed specifically for plant viroid RNA sampling. Our kits enable you to collect viral RNA from mature leaves or flower without extracting any cannabinoids, which makes samples legal to mail back to our lab from anywhere in the world. We have validated this specifically on plants showing symptoms of HLVd infection in a fully operational licensed commercial cultivation facility. Our test can specifically and sensitively distinguish HLVd from other plant pathogens, even other viroids, that might be causing symptoms of stunted growth.
Newly infected plants may only contain detectable virus in part of the plant. You may need to collect multiple samples from different sides of the same plant to ensure a thorough screening.
We include an internal positive control in every test reaction and negative controls in every batch of tests we run. The positive control provides a known reference so that we can obtain a conclusive result for the specific presence or absence of HLVd RNA in a sample. A positive control ensures that negative test results don’t simply arise from poor sample quality or other factors that could prevent successful detection. Negative controls (a “blank” test that doesn’t contain sample material) ensure we don’t generate erroneous false positives due to potential contamination. This extra information is incredibly useful when screening for low levels of HLVd.
Detailed instructions along with a sample collection form will be sent to you with the sample collection kits so you can properly document all necessary information. We track samples submitted to our lab using the sample collection form, which should contain the same contact information used when purchasing testing here on our website. Please contact us or include a note with your order if you would like results sent to a different email address than used for payment.