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Genomic Data Analysis

Comparative Genomics

Find Genetic Markers For Cannabis and Hemp Cultivars

It can be difficult to understand exactly how one cultivar is related to another at the genetic level.

Summary data generated by current sequencing providers showing broad statistics and phylogenetic relationships can be useful at first glance to get a general sense of what your data contains. However, when you look more closely, those summaries don’t provide actionable information that can be used to improve your breeding program in a meaningful way.

‚ÄčUsing an extensive repertoire of techniques, Delta Leaf can go beyond generating the types of charts and graphs typically provided in the report for a single sample, like those shown below. We provide more than just data, we turn your data into useable information that you can act on today.

We’ve compared numerous genome assemblies to identify a broad collection of genes of interest including:

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Cannabinoid synthases  

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Terpene synthases
E
Cannabinoid & terpene precursor enzymes (MEV and MEP pathways)
E
Flavonoid synthases
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and many more.

Our investigation isn’t limited to the above listed genes and we always consider the full dataset when conducting a data analysis procedure.

We can dive deeper into your raw data to uncover unique variants in your cultivars. This information can be used to develop phenotype correlation experiments, as well as for designing custom genotyping assays to identify phenotypes with early detection screening.

Delta Leaf has developed data mining pipelines to map quantitative trait loci using SNPs and other genetic variants by comparing multiple samples. This type of analysis can be useful to identify:

  • Potential breeding pairs‚Äč
  • Inherited markers (e.g. parental vs. offspring similarities and differences)
  • Shared markers among cultivars with true-breeding traits (e.g. pest resistance, morphology, chemotype, etc.)
  • ‚ÄčAnd more…‚Äč

Custom Genotyping Assays

Do you have a cultivar with a true breeding trait that shows up later in development, such as during the flowering stage?

Using your existing sequencing or microarray data, we can identify genetic markers for true-breeding traits and design custom genotyping assays to detect them at the seedling stage.

‚ÄčContact us for additional information about developing custom genotyping assays for your cultivars. Additional breeding and/or data collection may be required.