HOW TO COLLECT DNA SAMPLES

Before Collecting Samples

Please read the entire instructions carefully before taking your samples. Download a printer friendly version here.

Order kits. Then, gather the required tools and supplies (see list below).

Do not use dead, yellow or brown leaves. The ideal time to sample is right after the first true (serrated) leaves emerge, usually around 7-10 days post-germination. Please DO NOT use true leaves, use cotyledons (round leaves) to get the best sample.

Water your plants 24-48 hours before collecting samples.

Label plants and DNA Collection Kits before starting to ensure each sample is tracked back to the correct plant.

Avoid cross-contamination during sample collection by wiping your tools with alcohol before and after working with each plant.

You Will Need

Nitrile or Latex gloves

Nitrile or Latex gloves
To help to reduce the risk of cross-contamination

Tweezers

For plucking leaves

Scissors

Scissors
To cut leaf samples from plant

Alcohol (70% isopropyl or ethyl)

Alcohol (70% isopropyl or ethyl)
To wipe clown tools before and after each plant DNA sample is collected

+

Plus

E

A clean working surface

To document and collect DNA samples

E

A blunt object

Sturdy enough to crush leaves (e.g. a coin, marker or key)

E

A sample collection form

To track your samples and ensure correct labeling. You can avoid writing out names by hand, (and save time, especially for large orders), by accessing an editable online version of the form here, then emailing or sharing the form when you submit your samples.

Click on the image above for a printable version of the form.

Collecting Plant DNA Samples

Step 1

Identify and cut a cotyledon from your plant. Place the cotyledon between the plastic cover and filter paper.

STEP 2

Close the plastic cover and place on a solid surface.

STEP 3

Use a coin or blunt object to crush your sample through the plastic film. Continue until the leaf soaks fluid completely through both sides of the filter paper, leaving a dark green spot the size of your fingernail.