HOW TO COLLECT DNA SAMPLES
Before Collecting Samples
Do not use dead, yellow or brown leaves. The ideal time to sample is right after the first true (serrated) leaves emerge, usually around 7-10 days post-germination. Please DO NOT use true leaves, use cotyledons (round leaves) to get the best sample.
Water your plants 24-48 hours before collecting samples.
Label plants and DNA Collection Kits before starting to ensure each sample is tracked back to the correct plant.
Avoid cross-contamination during sample collection by wiping your tools with alcohol before and after working with each plant.
You Will Need
Nitrile or Latex gloves
To help to reduce the risk of cross-contamination
To cut leaf samples from plant
Alcohol (70% isopropyl or ethyl)
To wipe clown tools before and after each plant DNA sample is collected
A clean working surface
A blunt object
A sample collection form
Collecting Plant DNA Samples
Identify and cut a cotyledon from your plant. Place the cotyledon between the plastic cover and filter paper.
Close the plastic cover and place on a solid surface.
Use a coin or blunt object to crush your sample through the plastic film. Continue until the leaf soaks fluid completely through both sides of the filter paper, leaving a dark green spot the size of your fingernail.
Remove leaf remnants leaving behind only what was absorbed by the paper.
Place sample inside the envelope then clean your tools, including the object used to crush your sample
Please do not seal the envelopes. Simply close the flap and use rubber bands to group batches of envelopes when you’re done.